Water samples were obtained using 30 l Niskin bottles. Duplicate samples (250 ml) for MZ were preserved with 10% (final concentration) acid Lugol’s solution in amber glass bottles.
Methodology:
Methodology_Type: Lab
Methodology_Description:
MZ samples were returned to Horn Point Laboratory for enumeration of > 20 μm MZ (> ~15 μm when fixed with acid Lugol’s) using settling slides and a Nikon Eclipse TE 2000-U inverted microscope. In our data sheets an “0” indicates <40 cells/liter. A SPOT v 4.0 RT camera and SPOT diagnostic software were used to record images and to measure cells. In accordance with earlier MZ studies in the Bering Sea, ciliates smaller than 20 μm and mixotrophic dinoflagellates as well heterotrophic dinoflagellates were enumerated. Onboard, we examined “fresh” samples, either live or fixed with 1% glutaraldehyde with transmitted light and epifluorescence microscopy to look for the presence or absence of plastids. Our notes from the cruise facilitated in determination of which ciliates and dinoflagellates were photosynthetic (had chlorophyll fluorescence) and which were strictly heterotrophic (no chlorophyll fluorescence except associated with algae in food vacuoles). Separate data sheets are presented for ciliates, dinoflagellates and “other” microzooplankton
Process_Step:
Process_Description:
Skeleton metadata record created. New filename: B55_Stoecker_uZoop_Abundance.xml
Process_Date: 20091028
Metadata_Reference_Information:
Metadata_Date: 20091028
Metadata_Contact:
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Metadata_Standard_Name:
FGDC Biological Data Profile of the Content Standard for Digital Geospatial Metadata
Metadata_Standard_Version: FGDC-STD-001.1-1999
Generated by mp version 2.9.7 on Wed Oct 28 14:58:17 2009